It isn't easy being green. It takes thousands of genes to build the photosynthetic machinery that plants need to harness sunlight for growth. And yet, researchers don't know exactly how these genes work.
 |
| To build the library, researchers grew tens of thousands of strains of algae in plastic plates. The project, which took nine years, allows researchers to explore genes involved in photosynthesis and other aspects of plant biology [Credit: Xiaobo Li et al. 2019] |
"The part of the plant responsible for photosynthesis is like a complex machine made up of many parts, and we want to understand what each part does," said Martin Jonikas, assistant professor of molecular biology at Princeton. "This library, we hope, will be one of the foundations that people will build on to make the next generation of discoveries."
Unlocking the role of each gene could allow researchers to engineer plants to grow more quickly, potentially meeting future world food needs. Plants could also potentially be altered to absorb more carbon dioxide, helping to address climate challenges.
The library, funded in large part through a grant from the National Science Foundation, consists of thousands of single-celled, pond-dwelling algae known as Chlamydomonas reinhardtii, or Chlamy for short. Each "book" in the library is a strain of Chlamy with a single mutation. The 62,000-plus mutant strains, housed at the University of Minnesota's Chlamydomonas Resource Center, cover more than 80 percent of Chlamy's genes.
Similar libraries have been made in other single-celled organisms, such as yeast, but this is the first such endeavor for any single-celled photosynthetic organism. The rapid growth of single-celled organisms makes them valuable as research tools.
"Because this algal species is often used as a model to understand a wider range of biological processes, this library will be an important resource," said Karen Cone, a program director at the National Science Foundation, which was the primary funder for this research. "The partnership between the Jonikas group and the Chlamydomonas Resource Center enhances community accessibility to this valuable resource, which in turn will enable new discoveries, especially in one of NSF's research priority areas, 'Understanding the Rules of Life.'"
Due to various challenges inherent in Chlamy's genome, the project took nine years to complete. Throughout the project, researchers used robots to keep generations of cells alive by changing the nutrient-rich liquid media in which they live.
The project started in 2010 while Jonikas and his team were at the Carnegie Institution for Science on the Stanford University campus, and was completed at Princeton where the Jonikas laboratory moved in 2016. The project was a collaboration with Arthur Grossman, a senior staff scientist at Carnegie and with the Chlamydomonas Resource Center run by Paul Lefebvre, a professor of plant and microbial biology at the University of Minnesota.
The ability to observe a Chlamy cell with just one defective gene among all the other functioning genes allows researchers to figure out what that gene does. For example, if the cell has trouble moving, then the defective gene's function mostly likely involves governing movement.
Jonikas compared the Chlamy mutant library to a library containing thousands of copies of a manual for constructing a car, with each copy missing a different section. No matter which manual was used, the resulting car would be missing a part, making it unable to operate as expected.
"The horn might not work, or the steering wheel might not turn," Jonikas said. "Then you would know that the missing section contained the instructions for that part of the car."
The library enables researchers to test multiple mutant Chlamy strains at once because each mutation is labeled with a unique "DNA barcode." For the current study, investigators placed thousands of Chlamy strains in a single flask and exposed them to light. Strains that failed to grow were more likely to contain a gene involved in photosynthesis.
One of the newly identified genes is CPL3, which is thought to play a role in accumulating the protein "parts" of the photosynthetic machinery. The team is now exploring whether the gene helps the algae adjust their photosynthetic activity to changes in sunlight levels.
The mutant library can enable studies in other areas of plant biology, such as intracellular communication and Chlamy's ability to paddle around its environment using a tail-like cilium.
Xiaobo Li, the study's first author, was a postdoctoral researcher at Princeton when the team completed the library. "It is our hope that the Chlamydomonas mutant library and the genes identified will lead to numerous fundamental discoveries in photosynthesis, cell motility and many other processes," Li said.
Weronika Patena, a senior bioinformatics analyst in the Jonikas laboratory, wrote computer programs to analyze large amounts of data to identify genes most likely to be involved in photosynthesis. "I believe the success of this project will greatly accelerate research into photosynthesis and other processes for which Chlamy is a good model, and provide a lot of value to the scientific community," she said.
Source: Princeton University [March 18, 2019]
from The Archaeology News Network https://ift.tt/2HycwVP
Breaking News: Algal library lends insights into genes for photosynthesis - News Paper
0 komentar:
Post a Comment